| Product Name | BNP antibody [50E1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [50E1] to BNPBrain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides, see fig. 18). BNP is a 32 amino acid peptide with disulfide bond between the cystein residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity |
| Immunogen | Synthetic peptide - Human BNP (whole molecule) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 50E1 |
| Host Species | Mouse |
| Tested Applications | EIAELISAWB |
| For ELISA: Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [24C5] to BNP (GTX14699). For EIA: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Brain natriuretic peptide32, BNP 32, NPPB protein, BNP32, Brain natriuretic peptide 32, Natriuretic peptides B, Natriuretic peptide brain type, BNP, NPPB, Natriuretic peptide precursor B, Gamma brain natriuretic peptide |
|---|---|
| Target | BNP |
| Application Notes | For ELISA: Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [24C5] to BNP (GTX14699). For EIA: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH7.4, containing 0.1% Sodium azide |
