| Product Name | BRCA1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to BRCA1BRCA 1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyperphosphorylation during late G1 and S phases of the cell cycle and is transiently dephosphorylated early after M phase. BRCA 1protein alters in a qualitative and quantitative manner during cell cycle progression. The amount of BRCA protein is highest during S phase and remains elevated toward G2 / M, before it declines in early G1 phase. Inherited loss of BRCA 1 function confers an increased susceptibility for both breast and ovarian cancer. |
| Immunogen | Synthetic peptide: amino acids 1847-1863 of Human BRCA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IP,WB |
| Immunofluorescence: assay dependent. Immunoprecipitation (with protein A): use at a concentration of 10μg / mg of lysate (co-immunoprecipitates EGFR). Western Blotting: use at a concentration of 15 - 30μg / ml for 2h at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 220 kDa, which corresponds to the predicted molecular weight of BRCA 1. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 113705, RNF53, BRCA1, BRCAI, 672, PSCP, BRCC1, P38398, IRIS |
|---|---|
| Target | BRCA1 |
| Tissue Specificity | This antibody also cross reacts with a 150 kDa protein which is most likely EGFR (Epidermal Growth Factor Receptor). |
| Cellular Localization | Nuclear |
| Application Notes | Immunofluorescence: assay dependent. Immunoprecipitation (with protein A): use at a concentration of 10μg / mg of lysate (co-immunoprecipitates EGFR). Western Blotting: use at a concentration of 15 - 30μg / ml for 2h at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 220 kDa, which corresponds to the predicted molecular weight of BRCA 1. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
