| Product Name | BRCA1 antibody [GLK-2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [GLK-2] to BRCA1GTX17251 stains 94% of ovarian carcinomas with a mutation in exon 11. However, staining is absent in 100% of tumors with mutations other than exon 11. |
| Immunogen | Synthetic peptide: LDSVALYQCQELDTYLIPQIPHSHY, corresponding to C terminal amino acids 1839-1863 of Human BRCA1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | GLK-2 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| IHC-P: 1/15 - 1/50 in an ABC method. An incubation period of 30-60 minutes at room temperature is recommended. Perform heat mediated antigen retrieval with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human,Dog,Monkey,Chimpanzee,Gorilla,Bovine |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | 113705, RNF53, BRCA1, BRCAI, 672, PSCP, BRCC1, P38398, IRIS |
|---|---|
| Target | BRCA1 |
| Cellular Localization | Nuclear |
| Application Notes | IHC-P: 1/15 - 1/50 in an ABC method. An incubation period of 30-60 minutes at room temperature is recommended. Perform heat mediated antigen retrieval with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: Sodium Azide. Constituents: Tissue culture supernatant |
