Application
Sample (30 µg of whole cell lysate) A: HeLa 5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | BRCA2 antibody [5F6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [5F6] to BRCA2BRCA2-5F6 recognizes the BRCA2 gene product, a 390kDa nuclear protein. The BRCA2 gene was originally isolated based on its importance in the predisposition of women to early-onset breast and ovarian cancers, suggesting mutations affect its tumor suppressor-like activity. Targeted disruption of the BRCA2 gene in mice and binding of Rad51 to its carboxyl terminus have indicated an involvement in DNA recombination and double strand break repair. |
| Immunogen | Amino acids 2336-2478 of human BRCA2 expressed in E. coli. BRCA2-P400. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5F6 |
| Host Species | Mouse |
| Tested Applications | IHC,IHC-F,IHC-P,WB |
| Do not boil samples for longer than two minutes. For WB: Use at a concentration of 1-5 µg/ml. Predicted molecular weight: 380 kDa. Optimal dilutions/concentrations should be determined by the reseracher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 114480, FACD, FANCB, P51587, FANCD1, BRCC2, BRCA2, FANCD, 675, FAD1, FAD |
|---|---|
| Target | BRCA2 |
| Molecular Weight(MW) | 390 kDa (note) |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
Sample (30 µg of whole cell lysate) A: HeLa 5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Application Notes | Do not boil samples for longer than two minutes. For WB: Use at a concentration of 1-5 µg/ml. Predicted molecular weight: 380 kDa. Optimal dilutions/concentrations should be determined by the reseracher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, no preservative added. |
