Application
Cell lysates of HeLa(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | BRCC36 antibody [AT3B1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT3B1] to BRCC36BRCC36 (BRCA1/BRCA2-containing complex, subunit 36) is a holoenzyme complex containing BRCA1, BRCA2. BRCC36 is an ubiquitin E3 ligase complex that has a role in DNA repair and enhances cellular survival following DNA damage. This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint. |
| Immunogen | Recombinant BRCC36 (1-316aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AT3B1 |
| Host Species | Mouse |
| Tested Applications | ELISA,WB |
| Western Blot: Use at 1:1000 ~ 2000. Optimal working concentrations should be determined experimentally by the end user. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C6.1A, BRCA1/BRCA2containing complex subunit 3, chromosome X open reading frame 53, BRCA1/BRCA2containing complex subunit3, chromosome X open reading frame53, BRCC3, BRCA1/BRCA2-containing complex subunit 3 |
|---|---|
| Target | BRCC36 |
| Cellular Localization | Localizes at sites of DNA damage at double-strand breaks. |
| Light Chain Type | kappa |
Application
Cell lysates of HeLa(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | Western Blot: Use at 1:1000 ~ 2000. Optimal working concentrations should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide |
