| Product Name | BrdU antibody [B33.1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [B33.1] to BrdUThe immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. |
| Immunogen | Chemical / Small Molecule conjugated to BSA. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | B33.1 |
| Host Species | Mouse |
| Tested Applications | IHC-F,IHC-P |
| Formalin-fixed, paraffin-embedded tissue sections. For a three-stepavidin-biotin complex system, a dilution of 1:50 to 1:100 may be used as a guideline. We recommendan incubation time and temperature of 30 minutes at 37degC for this antibody.Enzymatic predigestion with proteolytic enzymes is recommended. Subsequently incubate theslides for 45 minutes in 95% formamide in 0.15M trisodium citrate at 70°C for the partial denaturationof double stranded DNA. | |
| Species Reactivity | Other |
| Concentration | 0.2mg/ml |
| Purification | Unpurified |
| Gene Synonyms | Bromodeoxyuridine antibody BUdr antibody |
|---|---|
| Target | BrdU |
| Tissue Specificity | This antibody recognizes BrdU in single stranded DNA, free BrdU or BrdU coupled to protein carrier. The antibody binds to the cells which have incorporated BrdU into their DNA during the S-phase of the cell cycle. It labels BrdU in the nuclei of a wide range of cell types. |
| Light Chain Type | kappa |
| Application Notes | Formalin-fixed, paraffin-embedded tissue sections. For a three-stepavidin-biotin complex system, a dilution of 1:50 to 1:100 may be used as a guideline. We recommendan incubation time and temperature of 30 minutes at 37degC for this antibody.Enzymatic predigestion with proteolytic enzymes is recommended. Subsequently incubate theslides for 45 minutes in 95% formamide in 0.15M trisodium citrate at 70°C for the partial denaturationof double stranded DNA. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.2% BSA and 0.09% sodium azide |
