Application
Immunohistochemistry of paraffin-embedded sections (chick embryo) usingApplication
Flow cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM human acute lymphoblastic leukemia cell line using purified anti-5-bromodeoxyuridin (MoBu-1) (detection by Goat anti-mouse IgG1 FITC). The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.| Product Name | BrdU antibody [MoBu-1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MoBu - 1] to BrdU - Proliferation MarkerImmunocytochemical labeling with the antibody provides a simple and rapid method for estimation of the cell proliferation. The antibody can be used for histochemistry on frozen tissue sections, formalin-fixed, paraffin-embedded sections as well as in immunofluorescence and FACS. |
| Immunogen | 5-bromodeoxyuridine conjugated with hemocyanine (isolated from Helix pomatia ). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MoBu-1 |
| Host Species | Mouse |
| Tested Applications | FACS,ICC/IF,IHC-P |
| FACS:1-2 μg/ml | |
| Species Reactivity | Other |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Target | BrdU |
|---|---|
| Tissue Specificity | This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. The reaction shows a clear, nuclear confined speckled pattern. |
Application
Immunohistochemistry of paraffin-embedded sections (chick embryo) using
Application
Flow cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM human acute lymphoblastic leukemia cell line using purified anti-5-bromodeoxyuridin (MoBu-1) (detection by Goat anti-mouse IgG1 FITC). The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.| Application Notes | FACS:1-2 μg/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
