Application
WB analysis of various cell lysates using BRG1 antibody [4E11-E11-G11] at a dilution of 1:1000.Application
IP analysis was perform with HeLa lysates and either control IgG or BRG1 antibody [4E11-E11-G11]. The precipitates were detected by the same antibody.| Product Name | BRG1 antibody [4E11-E11-G11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [4E11-E11-G11] to BRG1The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] |
| Immunogen | Purified recombinant human BRG1 protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4E11-E11-G11 |
| Host Species | Mouse |
| Tested Applications | ID,IP,WB |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SNF2LB , BAF190A , SNF2L4 , MRD16 , SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 , RTPS2 , hSNF2b , BAF190 , SMARCA4 , SNF2 , SWI2 |
|---|---|
| Target | BRG1 |
Application
WB analysis of various cell lysates using BRG1 antibody [4E11-E11-G11] at a dilution of 1:1000.
Application
IP analysis was perform with HeLa lysates and either control IgG or BRG1 antibody [4E11-E11-G11]. The precipitates were detected by the same antibody.| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol. |
