Application
Immunostaining of Brg1 by BRG-01 antibody.A - Brg1-negative SW13 cells were transfected with Brg1-expression plasmid and analysed by Western blotting for Brg1 expression.B - HEK293 cytoplasmic (cyt) and nuclear (nuc) lysates were analysed by Western blotting for Brg1 presence.| Product Name | BRG1 antibody [BRG-01] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [BRG-01] to Brg1Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-like chromatin-remodeling complexes that enable access of regulatory and effector proteins in transcription, DNA repair and DNA replication. Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumour supression.Entrez Gene: 19p13.2 |
| Immunogen | Bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | BRG-01 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at 10 µg/mL. Positive Control: HEK293 cells | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BAF190, BRG1, SNF2-BETA, SNF2L4, SNF2LB, hSNF2b |
|---|---|
| Target | Brg1 |
| Tissue Specificity | The antibody BRG-01 recognizes an epitope within C-terminal part of human Brg1, a 205 kDa catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes. |
Application
Immunostaining of Brg1 by BRG-01 antibody.A - Brg1-negative SW13 cells were transfected with Brg1-expression plasmid and analysed by Western blotting for Brg1 expression.B - HEK293 cytoplasmic (cyt) and nuclear (nuc) lysates were analysed by Western blotting for Brg1 presence.| Application Notes | WB: Use at 10 µg/mL. Positive Control: HEK293 cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
