Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] diluted at 1:1000.Application
BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. BRG1 antibody [GT2712] diluted at 1:250.Application
BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. BRG1 antibody [GT2712] diluted at 1:250.Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] diluted at 1:1000.Application
BRG1 antibody [GT2712] detects BRG1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRG1 protein stained by BRG1 antibody [GT2712] diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.| Product Name | BRG1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT2712] to BRG1 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4)The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human BRG1. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT2712 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, BovineChicken (83%), Bovine (85%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BAF190 antibody BAF190A antibody BRG1 antibody [GT2712] antibody BRG1 antibody MRD16 antibody RTPS2 antibody SMARCA4 antibody SNF2 antibody SNF2L4 antibody SNF2LB antibody SWI/SNF related matrix associated actin dependent re |
|---|---|
| Target | BRG1 |
| Molecular Weight(MW) | 185 kDa (note) (7) |
| Cellular Localization | Nucleus |
Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] diluted at 1:1000.
Application
BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. BRG1 antibody [GT2712] diluted at 1:250.
Application
BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. BRG1 antibody [GT2712] diluted at 1:250.
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] diluted at 1:1000.
Application
BRG1 antibody [GT2712] detects BRG1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRG1 protein stained by BRG1 antibody [GT2712] diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.| Positive Control | K562 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
