Application
Western blot analysis using PTK6 monoclonal antiobdy against Hela (1), A549 (2) and MCF-7 (3) cell lysate.| Product Name | Brk antibody [2H12B8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2H12B8] to BrkThe protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
| Immunogen | The immunogen corresponding to human PTK6 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 2H12B8 |
| Host Species | Mouse |
| Tested Applications | ELISA,WB |
| ELISA: 1/10000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BRK |
|---|---|
| Target | PTK6 |
| Molecular Weight(MW) | 52 kDa (note) |
| Cellular Localization | Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors. |
Application
Western blot analysis using PTK6 monoclonal antiobdy against Hela (1), A549 (2) and MCF-7 (3) cell lysate.| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
