| Product Name | BTK (phospho Ser180) antibody [3i5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3i5] to BTK ( phospho S180 )Brutons tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain, Src homology SH3 and SH2 domains. Btk plays an important role in B cell development. Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane-located Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. The activation of Btk is negatively regulated by PKCbeta through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation. The PKC/Btk inhibitory signal is likely to be a key determinant of the B-cell receptor signaling threshold to maintain optimal Btk activity. |
| Immunogen | Synthetic peptide conjugated to KLH. |
| Modification | p-Ser180 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | 3i5 |
| Host Species | Mouse |
| Tested Applications | IP,WB |
| IP: Use at a dilution of 1/25. WB: Use at a dilution of 1/1000. Predicted molecular weight: 73 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | MGC126261, ATK, IMD1, AT, XLA, 695, Q06187, BPK, AGMX1, PSCTK1, 300300, MGC126262, BTK, BTK (phospho S180), BTK (phospho Ser180), BTK (pS180), BTK (pSer180), BTK phospho S180, BTK phospho Ser180, Phospho BTK (S180), Phospho BTK (Ser180), Phospho BTK (pS180 |
|---|---|
| Target | BTK |
| Cellular Localization | Cell Membrane and Cytoplasmic |
| Application Notes | IP: Use at a dilution of 1/25. WB: Use at a dilution of 1/1000. Predicted molecular weight: 73 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at -20°C. |
| Storage Buffer | Preservative: NoneConstituents: 50% Glycerol, 0.1mg/ml BSA, 150mM Sodium Chloride, 10mM HEPES. pH 7.5 |
