| Product Name | BUB1 antibody [14H5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [14H5] to BUB1Bub1 is a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in Bub1 have been associated with aneuploidy and several forms of cancer. |
| Immunogen | Recombinant human BUB1/CFA |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 14H5 |
| Host Species | Mouse |
| Tested Applications | ICC/IF,IP |
| Use at 1μg/ml | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | BUB1 budding uninhibited by benzimidazoles 1 homolog, BUB1A, BUB1L, Budding uninhibited by benzimidazoles 1, hBUB1, Mitotic checkpoint serine/threonine protein kinase BUB1, Mitotic spindle checkpoint kinase, Putative serine/threonine protein kinase, |
|---|---|
| Target | BUB1 |
| Tissue Specificity | Human BUB1. Not tested for reactivity with rodents or other vertebrates |
| Cellular Localization | Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpo |
| Light Chain Type | kappa |
| Application Notes | Use at 1μg/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. For extended storage, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS + 0.1% sodium azide |
