Application
Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BUB1BApplication
Immunohistochemical staining of paraffin-embedded breast tissue using anti-BUB1B mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-BUB1Bmouse monoclonal antibody. ( Dilution 1:50)| Product Name | BUB1B antibody [10E7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [10E7] to BUB1BEssential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. |
| Immunogen | Protein expressed in 293T cell transfected with human BUB1B expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10E7 |
| Host Species | Mouse |
| Tested Applications | ICC/IF,IHC,IHC-P,WB |
| IHC 1:50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bub1A, BUB1beta, BUB1B, hBUBR1, MAD3L, SSK1, BUBR1 |
|---|---|
| Target | BUB1B |
| Tissue Specificity | is specific for human BUB1B. |
| Cellular Localization | Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5. |
Application
Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BUB1B
Application
Immunohistochemical staining of paraffin-embedded breast tissue using anti-BUB1B mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-BUB1Bmouse monoclonal antibody. ( Dilution 1:50)| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
