Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.Application
HEK293T cells transfected with either RC205844 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BUB1B antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.Application
Flow cytometric Analysis of Jurkat cells, using anti-BUB1B antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BUB1BApplication
Flow cytometric Analysis of Hela cells, using anti-BUB1B antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).| Product Name | BuB1B antibody [6E3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6E3] to BuB1BEssential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. |
| Immunogen | Full length human recombinant protein of human BUB1B (NP_001202) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6E3 |
| Host Species | Mouse |
| Tested Applications | FACS,ICC/IF,WB |
| Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human,Dog,Monkey,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bub1A, BUB1beta, BUB1B, hBUBR1, MAD3L, SSK1, BUBR1 |
|---|---|
| Target | BuB1B |
| Tissue Specificity | is specific for human BUB1B (NP_001202). |
| Cellular Localization | Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5. |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.
Application
HEK293T cells transfected with either RC205844 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BUB1B antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.
Application
Flow cytometric Analysis of Jurkat cells, using anti-BUB1B antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BUB1B
Application
Flow cytometric Analysis of Hela cells, using anti-BUB1B antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).| Application Notes | Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
