Application
Humna CRP positive serum was separated on SDS-PAGE and probed with Monoclonal AB to C-Reactive Protein (MO) CL:CRP-8 . The antibody was developed with Biotin APA M Fab ads Hu/Rat conjugate, Alkaline Phosphatase ExtrAvidin and a NBT/BCIP substrate. Antibody dilution 1/400| Product Name | C Reactive Protein antibody [CRP-8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CRP - 8] to C Reactive ProteinC Reactive Protein is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is a pentraxin (cyclic pentameric protein) compound of five identical nonglycosylated subunits of 206 amino acids each (m.w. 24 kDa), that are bound noncovalently to form the physiologic CRP molecule (m.w. 117.5 kDa). C Reactive Protein mediates activities associated with preimmune nonspecific host resistance. It is opsonic, an initiator of the classical complement cascade and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. Studies have revealed that among other markers of inflammation, CRP shows the strongest association with cardiovascular events. Many clinical studies demonstrated that coronary mortality among patients with unstable angina and elevated CRP is significantly higher comparing with the patients without elevated CRP. Measurements of C-reactive protein (hsCRP) in the patients with ischemic heart disease provide a novel method for detecting individuals at high risk of plaque rapture. |
| Immunogen | Full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CRP-8 |
| Host Species | Mouse |
| Tested Applications | Dot,ELISA,WB |
| Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/6000 for indirect ELISA using freshly prepared human CRP at 10 μg/ml for coating. WB: Use at an assay dependent dilution. Predicted molecular weight (following denaturation and reduction): 24 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CRP, 123260, PTX1, MGC88244, C Reactive Protein, P02741, 1401 |
|---|---|
| Target | C Reactive Protein |
| Tissue Specificity | Does not cross react with human serum amyloid P component (SAP), human haptoglobin, human alpha-1-acid glycoprotein and human IgG, nor with C Reactive Protein from Limulus. Cross reacts with both native and reduced forms of C Reactive Protein. |
| Cellular Localization | Secreted |
Application
Humna CRP positive serum was separated on SDS-PAGE and probed with Monoclonal AB to C-Reactive Protein (MO) CL:CRP-8 . The antibody was developed with Biotin APA M Fab ads Hu/Rat conjugate, Alkaline Phosphatase ExtrAvidin and a NBT/BCIP substrate. Antibody dilution 1/400| Application Notes | Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/6000 for indirect ELISA using freshly prepared human CRP at 10 μg/ml for coating. WB: Use at an assay dependent dilution. Predicted molecular weight (following denaturation and reduction): 24 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide |
