Application
NIH 3T3 cell lysates(30?g) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C/EBP-? antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | C/EBP beta antibody [47A1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [47A1] to C/EBP-BetaCCAAT/enhancer-binding proteins (C/EBPs) are a family of basic leucine zipper (bZIP) DNA binding proteins that play important roles in normal cellular differentiation and function in a variety of tissues. The C/EBP family is critical for innate immunity and inflammatory response, and consists of five core members: C/EBPalpha, C/EBP?, C/EBP?, C/EBPepsilon, and C/EBPgamma . Among these proteins, C/EBPbeta is usually highly expressed in liver, intestine, lung and adipose. C/EBPbeta has three isoforms, two activator, (LAP* and LAP) and a repressor (LIP). These isoforms are differentially translated from the same mRNA. The expression of these isoforms is developmentally and hormonally regulated. |
| Immunogen | Recombinant human C/EBP-beta (1-271 aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 47A1 |
| Host Species | Mouse |
| Tested Applications | ELISA,WB |
| Western Blot: Use at 1:500 ~ 2,000. Optimal working concentrations should be determined experimentally by the end user. |
|
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CRP2, LAP, IL6DBP, interleukin 6dependent DNAbinding protein, MGC32080,C/EBPbeta, interleukin6dependent DNAbinding protein, TCF5, beta), NFIL6, liverenriched transcriptional activator protein, CEBPB, CCAAT/enhancer binding protein (C/EBP) beta, MGC32080,C/ |
|---|---|
| Target | C/EBP-Beta |
| Light Chain Type | kappa |
Application
NIH 3T3 cell lysates(30?g) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C/EBP-? antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | Western Blot: Use at 1:500 ~ 2,000. Optimal working concentrations should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
