Application
Western blot analysis of CA14 (arrow) using rabbit polyclonal CA14 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA14 gene.Application
Flow cytometric analysis of A375 cells using CA14 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CA14 antibody (N-term) in A375 cell line lysates (35ug/lane). CA14 (arrow) was detected using the purified Pab.| Product Name | CA14 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CA14 (N-term)Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 58~88 amino acids from the N-terminal region of human CA14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Carbonic anhydrase XIV CA-XIV Carbonic anhydrase 14 CA14 Carbonic anhydrase14 Carbonate dehydratase XIV CAXIV UNQ690/PRO1335 CA XIV |
|---|---|
| Target | CA14 |
| Molecular Weight(MW) | 37.668 kDa (note) |
Application
Western blot analysis of CA14 (arrow) using rabbit polyclonal CA14 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA14 gene.
Application
Flow cytometric analysis of A375 cells using CA14 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CA14 antibody (N-term) in A375 cell line lysates (35ug/lane). CA14 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
