Application
CA5B antibody detects CA5B protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CA5B antibody diluted by 1:1000.| Product Name | CA5B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CA5B (carbonic anhydrase VB, mitochondrial)Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CA5B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rat, BovineRat (96%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | carbonic anhydrase VB mitochondrial CA-VB |
|---|---|
| Target | CA5B |
| Molecular Weight(MW) | 36 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
CA5B antibody detects CA5B protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CA5B antibody diluted by 1:1000.| Positive Control | AsPC-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
