Application
Anti-Cavalpha2delta4_(extracellular) - Western blot analysis of rat DRG (lanes 1 and 3) and PC12 cells (lanes 2 and 4): 1-2. Anti-Cavα2δ4 (extracellular) antibody, (1:200). 3-4. Anti-Cavα2δ4 (extracellular) antibody preincubated with the control antigen.Application
Anti-Cavalpha2delta4_(extracellular) - Expression of Cavalpha2delta4 in rat C6 cells Immunocytochemical staining of intact living rat brain glioma (C6) cells. A. Extracellular staining of cells with Anti-Cavα2δ4 (extracellular) antibody, (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342 (blue). C. Merged images of A and B.Application
Anti-Cavalpha2delta4_(extracellular) - Expression of Cavalpha2delta4 in rat DRG Immunohistochemical staining of rat DRG frozen section using Anti-Cavα2δ4 (extracellular) antibody, (1:100). A. Cavα2δ4 is expressed in DRG neurons. B. Nuclear staining using Hoechst 33342 as the counterstain. C. Merged images of A and B.| Product Name | CACNA2D4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CACNA2D4 |
| Immunogen | Peptide (C)SERPQEMGRLLGEADG, corresponding to amino acid residues 881-896 of mouse Cavα2δ4 (Accession Q5RJF7). Extracellular. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-FWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-F:1:50~1:100 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Cacna2d4 calcium channel voltage-dependent alpha 2/delta subunit 4 BE686333 5730412N02Rik |
|---|---|
| Target | CACNA2D4 |
Application
Anti-Cavalpha2delta4_(extracellular) - Western blot analysis of rat DRG (lanes 1 and 3) and PC12 cells (lanes 2 and 4): 1-2. Anti-Cavα2δ4 (extracellular) antibody, (1:200). 3-4. Anti-Cavα2δ4 (extracellular) antibody preincubated with the control antigen.
Application
Anti-Cavalpha2delta4_(extracellular) - Expression of Cavalpha2delta4 in rat C6 cells Immunocytochemical staining of intact living rat brain glioma (C6) cells. A. Extracellular staining of cells with Anti-Cavα2δ4 (extracellular) antibody, (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342 (blue). C. Merged images of A and B.
Application
Anti-Cavalpha2delta4_(extracellular) - Expression of Cavalpha2delta4 in rat DRG Immunohistochemical staining of rat DRG frozen section using Anti-Cavα2δ4 (extracellular) antibody, (1:100). A. Cavα2δ4 is expressed in DRG neurons. B. Nuclear staining using Hoechst 33342 as the counterstain. C. Merged images of A and B.| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-F:1:50~1:100 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
