Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CAD (Phospho-Thr456) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | CAD (phospho Thr456) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CAD (phospho Thr456)The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] |
| Immunogen | Peptide sequence around phosphorylation site of threonine 456 (P-I-T(p)-P-H) derived from Human CAD. |
| Modification | p-Thr456 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC: 1:50 - 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | carbamoyl-phosphate synthetase 2 aspartate transcarbamylase and dihydroorotase CAD |
|---|---|
| Target | CAD (phospho Thr456) |
| Tissue Specificity | The antibody detects endogenous levels of CAD only when phosphorylated at threonine 456. |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CAD (Phospho-Thr456) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
