Application
Immunocytochemical staining of DFF40 in K562 cells with CAD antibody at 10μg/ml.Application
Western blot analysis of DFF40 in Jurkat cell lysate with CAD antibody at (A) 0.5, (B) 1 and (C) 2 g/ml.| Product Name | CAD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CADApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (1,2). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively, (3-5). DFF45/ICAD is the inhibitory protein of DFF40/CAD (1,2,6) and forms complex with DFF40/CAD. Upon cleavage ofDFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death. |
| Immunogen | Peptide corresponding to amino acids 203 to 218of human DFF40 (4, 5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Western blot at 1:500 dilution.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Gene Synonyms | P27708 CAD 114010 790 |
|---|---|
| Target | CAD |
| Cellular Localization | Cytoplasmic and Nuclear. |
Application
Immunocytochemical staining of DFF40 in K562 cells with CAD antibody at 10μg/ml.
Application
Western blot analysis of DFF40 in Jurkat cell lysate with CAD antibody at (A) 0.5, (B) 1 and (C) 2 g/ml.| Positive Control | K562 or Jurkat whole cell lysate A 40 kDa band can be detected. |
|---|---|
| Application Notes | Western blot at 1:500 dilution.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at4 °C, stable for one year. |
| Storage Buffer | PBS with 0.02% sodium azide |
