Application
Immunocytochemical staining of DFF40 in Jurkat cells with CAD antibody at 5μg/ml.Application
Western blot analysis of DFF40/CAD in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40/CAD antibody at 1μg/ml.| Product Name | CAD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CADApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (1,2). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively, (3-5). DFF45/ICAD is the inhibitory protein of DFF40/CAD (1,2,6) and forms complex with DFF40/CAD. Upon cleavage ofDFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death. |
| Immunogen | Peptide corresponding to amino acids 147 to 164 of murineCAD (1). The sequence differs from human DFF40 by twoamino acids (3,4) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Western blot at 1:500 to 1:1000 dilution.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Gene Synonyms | CAD 69719 Cpad 2410008J01Rik AU018859 |
|---|---|
| Target | CAD |
| Cellular Localization | Cytoplasmic and Nuclear. |
Application
Immunocytochemical staining of DFF40 in Jurkat cells with CAD antibody at 5μg/ml.
Application
Western blot analysis of DFF40/CAD in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40/CAD antibody at 1μg/ml.| Positive Control | K562 or Jurkat whole cell lysate A 40 kDa band can be detected. |
|---|---|
| Application Notes | Western blot at 1:500 to 1:1000 dilution.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at4 °C, stable for one year. |
| Storage Buffer | PBS with 0.02% sodium azide |
