| Product Name | Calbindin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CB-955] antibodyCalbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and is related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. This Calbindin antibody is often used as a marker for cerebellum Purkinje cells. |
| Immunogen | Purified bovine kidney calbindin-D-28K |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CB-955 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| For IHC-P: Use at a dilution of 1:150-1:300 in ABC method. An incubation period of 30 minutes at room temperature is suggested. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 or enzymatic digestion prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogPigRatCatGoatGuinea PigRabbitSheepBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 793 P05937 Calbindin CALB1 114050 CALB MGC138959 |
|---|---|
| Target | Calbindin |
| Tissue Specificity | Antibody reacts specifically with calbindin-D-28K in brain and kidney tissues from human, cow, goat, pig, rabbit, dog, cat, rat, mouse, sheep, and guinea pig sources. It binds weakly to chicken calbindin-D-28K. |
| Cellular Localization | Cytoplasmic/Cell membrane |
| Positive Control | Kidney |
|---|---|
| Application Notes | For IHC-P: Use at a dilution of 1:150-1:300 in ABC method. An incubation period of 30 minutes at room temperature is suggested. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 or enzymatic digestion prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Ascites containing 0.09% sodium azide |
