Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Calcium Sensing Receptor antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft , using Calcium Sensing Receptor antibody at 1:500 dilution.| Product Name | Calcium Sensing Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Calcium Sensing Receptor (calcium-sensing receptor (hypocalciuric hypercalcemia 1, severe neonatal hyperparathyroidism))The protein encoded by this gene is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calcium Sensing Receptor. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cat, Pig, BovineMouse (93%), Rat (94%), Dog (95%), Cat (95%), Pig (95%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CAR antibody EIG8 antibody FHH antibody FIH antibody GPRC2A antibody HHC antibody HHC1 antibody MGC138441 antibody NSHPT antibody PCAR1 antibody CASR antibody parathyroid cell calcium-sensing receptor antibody parathyroid Ca(2+)-sensing recepto |
|---|---|
| Target | Calcium Sensing Receptor |
| Molecular Weight(MW) | 121 kDa (note) (2) |
| Cellular Localization | Cell membrane |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Calcium Sensing Receptor antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft , using Calcium Sensing Receptor antibody at 1:500 dilution.| Positive Control | 293T , A431 , H1299 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
