| Product Name | Caldesmon antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [C21] to CaldesmonMonoclonal anti-Caldesmon may be used for the study of the involvement of caldesmon in contraction, cell movement, shape change, exocytosis and endocytosis. Caldesmon is an actin interacting and calmodulin binding protein found in smooth muscle and other cell types. Caldesmon occurs in both a high molecular weight (120-150 kDa) and a low molecular weight (71-80 kDa) form, depending on the tissue in which it is located. Caldesmon plays a major role in the regulation of smooth muscle and non-muscle contractile events. |
| Immunogen | Purified chicken gizzard caldesmon. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | C21 |
| Host Species | Mouse |
| Tested Applications | DIDELISAICC/IFWB |
| DID: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. The minimum antibody titer of 1/2500 was determined in an ELISA with turkey gizzard or pig stomach caldesmon coated at 10 g/ml on polyvinyl microtiter plates. IF: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Caldesmon occurs in both a high molecular weight (120-150 KD) and a low molecular weight (60-80KD) form, depending on the tissue in which it is located. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanPigRatGerbilTurkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CALD1 Caldesmon 373965 |
|---|---|
| Target | Caldesmon |
| Tissue Specificity | Using immunoblot techniques Monoclonal anti-Caldesmon localizes both the high molecular weight form and the low molecular weight non-muscle form of caldesmon. Monoclonal anti-Caldesmon localizes the high molecular weight form of caldesmon in pig stomach and turkey gizzard, and the low molecular weight non-muscle caldesmon in gerbil fibroma cells (CCL-146), normal rat kidney cells (NRK), human EJ and Hut-11 cells and chicken embryo fibroblast cells. |
| Cellular Localization | Cytoplasmic |
| Application Notes | DID: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. The minimum antibody titer of 1/2500 was determined in an ELISA with turkey gizzard or pig stomach caldesmon coated at 10 g/ml on polyvinyl microtiter plates. IF: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Caldesmon occurs in both a high molecular weight (120-150 KD) and a low molecular weight (60-80KD) form, depending on the tissue in which it is located. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: Raw Ascites |
