Application
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using Calpain 1 antibody at 1:500 dilution.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Calpain 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE diluted at 1:1000| Product Name | Calpain 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Calpain 1 (calpain 1, (mu/I) large subunit)The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, Chicken, Sheep, Rhesus Monkey, BovineMouse (87%), Rat (87%), Xenopus laevis (86%), Pig (96%), Chicken (89%), Sheep (95%), Rhesus Monkey (99%), Bovine (95%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CANP antibody CANP1 antibody CANPL1 antibody muCANP antibody muCL antibody CAPN1 antibody calcium-activated neutral proteinase 1 antibody calpain mu-type antibody micromolar-calpain antibody cell proliferation-inducing gene 30 protein antibody CA |
|---|---|
| Molecular Weight(MW) | 82 kDa (note) (4) |
| Cellular Localization | Cytoplasm , Cell membrane |
Application
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using Calpain 1 antibody at 1:500 dilution.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Calpain 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE diluted at 1:1000| Positive Control | A431 , Jurkat |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
