Application
Calpain 1 antibody [N3C2], Internal detects Calpain 1 protein at cytoplasm on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Calpain 1 antibody [N3C2], Internal diluted at 1:500.Application
Calpain 1 antibody detects Calpain 1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 1 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of Calpain 1 protein from A431 whole cell extracts using 5 μg of Calpain 1 antibody [N3C2], Internal . Western blot analysis was performed using Calpain 1 antibody [N3C2], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Calpain 1 antibody at 1:500 dilution.Application
Immunofluorescence analysis of methanol-fixed A431, using Calpain 1 antibody at 1:200 dilution.| Product Name | Calpain 1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Calpain 1 (calpain 1, (mu/I) large subunit)The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Sheep, Rhesus Monkey, BovineRat (88%), Pig (96%), Sheep (96%), Rhesus Monkey (98%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CANP antibody CANP1 antibody CANPL1 antibody muCANP antibody muCL antibody CAPN1 antibody calcium-activated neutral proteinase 1 antibody calpain mu-type antibody micromolar-calpain antibody cell proliferation-inducing gene 30 protein antibody CA |
|---|---|
| Molecular Weight(MW) | 82 kDa (note) (4) |
Application
Calpain 1 antibody [N3C2], Internal detects Calpain 1 protein at cytoplasm on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Calpain 1 antibody [N3C2], Internal diluted at 1:500.
Application
Calpain 1 antibody detects Calpain 1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 1 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of Calpain 1 protein from A431 whole cell extracts using 5 μg of Calpain 1 antibody [N3C2], Internal . Western blot analysis was performed using Calpain 1 antibody [N3C2], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Calpain 1 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of methanol-fixed A431, using Calpain 1 antibody at 1:200 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
