Application
Calpain 2 antibody [N2C1], Internal detects Calpain 2 protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Calpain 2 antibody [N2C1], Internal dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calpain 2 antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: JurKat 7.5% SDS PAGE Calpain 2 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Calpain 2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Calpain 2 (calpain 2, (m/II) large subunit)The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calpain 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rabbit, Chicken, Sheep, Rhesus Monkey, BovineRat (89%), Pig (92%), Rabbit (92%), Chicken (85%), Sheep (93%), Rhesus Monkey (98%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CANP2 antibody CANPL2 antibody CANPml antibody FLJ39928 antibody mCANP antibody CAPN2 antibody calpain large polypeptide L2 antibody calpain 2 large [catalytic] subunit antibody calpain-2 large subunit antibody calpain 2 large subunit antibody |
|---|---|
| Molecular Weight(MW) | 80 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Cell membrane |
Application
Calpain 2 antibody [N2C1], Internal detects Calpain 2 protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Calpain 2 antibody [N2C1], Internal dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calpain 2 antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: JurKat 7.5% SDS PAGE Calpain 2 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Jurkat , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
