Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE Calpain 5 antibody diluted at 1:500Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Calpain 5 antibody at 1:500 dilution.Application
Immunofluorescence analysis of methanol-fixed HeLa, using Calpain 5 antibody at 1:200 dilution.| Product Name | Calpain 5 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Calpain 5 (calpain 5)Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Calpain 5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (86%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ46245 antibody HTRA3 antibody nCL-3 antibody CAPN5 antibody calpain htra-3 antibody calpain-5 antibody new calpain 3 antibody calpain 5 antibody |
|---|---|
| Molecular Weight(MW) | 73 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE Calpain 5 antibody diluted at 1:500
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Calpain 5 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Calpain 5 antibody at 1:200 dilution.| Positive Control | A431 , H1299 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
