Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 7.5% SDS PAGE Calpain-5 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Calpain-5 antibody at 1:100 dilution.| Product Name | Calpain-5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Calpain-5 (calpain 5)Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calpain-5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, BovineMouse (93%), Rat (92%), Xenopus laevis (81%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ46245 antibody HTRA3 antibody nCL-3 antibody CAPN5 antibody calpain htra-3 antibody calpain-5 antibody new calpain 3 antibody calpain 5 antibody |
|---|---|
| Target | Calpain-5 |
| Molecular Weight(MW) | 73 kDa (note) |
Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 7.5% SDS PAGE Calpain-5 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Calpain-5 antibody at 1:100 dilution.| Positive Control | 293T , A431 , H1299 , HeLaS3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
