Application
Western Blot: Calreticulin Antibody - Human kidney lysate.Application
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody - The Calreticulin antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.Application
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody - Immunofluorescence staining of Calreticulin in HCT15 colon cancer cells using . Secondary antibody was an Alexa Fluor 488.| Product Name | Calreticulin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Calreticulin - ER MarkerCalreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER). It is also a molecular chaperone and is involved in protein folding events in the ER. Calreticulin has also been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells. (2-3) Recently it has been shown that calreticulin is induced in response to various types of cell stress including amino acid deprivation. (1) Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and crucially participate in the coordination of these functions by the cell.(2-3) |
| Immunogen | A fusion protein to mouse calreticulin. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| For WB: Use at a dilution of 1:1,000 (ECL). By Western Blot a band of ~ 55 kDa is seen. For ICC: Use at a dilution of 1:250. For IHC: Use at a dilution of 1:50. For IF: Use at dilution of 1:50. For IP: Use at a dilution of 1:300. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRatHamster |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Calr 12317 Calregulin cC1qR 811 RO Calreticulin P27797 SSA 109091 CRT |
|---|---|
| Target | Calreticulin |
| Tissue Specificity | This antibody is specific for calreticulin. |
| Cellular Localization | Endoplasmic reticulum |
Application
Western Blot: Calreticulin Antibody - Human kidney lysate.
Application
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody - The Calreticulin antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
Application
Immunocytochemistry/Immunofluorescence: Calreticulin Antibody - Immunofluorescence staining of Calreticulin in HCT15 colon cancer cells using . Secondary antibody was an Alexa Fluor 488.| Application Notes | For WB: Use at a dilution of 1:1,000 (ECL). By Western Blot a band of ~ 55 kDa is seen. For ICC: Use at a dilution of 1:250. For IHC: Use at a dilution of 1:50. For IF: Use at dilution of 1:50. For IP: Use at a dilution of 1:300. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles |
| Storage Buffer | Phosphat-buffered saline containing 0.05% sodium azide |
