Application
Western blot analysis of Calreticulin: Lane 1: HeLa (Heat Shocked) Lane 2: VeroApplication
Immunohistochemistry analysis of human uterus tissue stained with Calreticulin, mAb (FMC 75) at 10µg/ml.| Product Name | Calreticulin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [FMC 75] to CalreticulinCalreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. This protein was originally identified in SR membranes and plays a minor role in calcium storage in skeletal and cardiac muscle SR. Calreticulin is also known as calregulin, CRP55, CaBP3, calsequestrin-like protein and Ro/SS-A antigen. Calreticulin binds calcium with low affinity and high capacity, however it also exhibits a single high affinity binding site. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of calreticulin and other resident ER proteins including glucose regulated protein 78 (GRP78), GRP94 and protein disulfide isomerase (PDI). This sequence is responsible for the retention of newly synthesized proteins within the ER lumen. This retention is reported to be mediated by a KDEL receptor. Recent reports indicate that calreticulin can act as a modulator of the regulation of gene transcription by nuclear hormone receptors and may also act as a molecular chaperone. |
| Immunogen | Calreticulin-maltose binding fusion protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | FMC 75 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCWB |
| Recommended dilutions: FACS: 1/100. ICC: Use at an assay dependent dilution. IHC: Use at a concentration of 2 μg/ml. IP: Use at a concentration of 12.5 μg/ml. WB: Use at a concentration of 0.5 μg/ml. Predicted molecular weight: 48 kDa. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMonkeyHamster |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | CRT FLJ26680 RO SSA cC1qR |
|---|---|
| Target | Calreticulin |
| Cellular Localization | Endoplasmic reticulum and Secreted. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes. |
| Light Chain Type | kappa |
Application
Western blot analysis of Calreticulin: Lane 1: HeLa (Heat Shocked) Lane 2: Vero
Application
Immunohistochemistry analysis of human uterus tissue stained with Calreticulin, mAb (FMC 75) at 10µg/ml.| Positive Control | HeLa heat shocked cell lysate |
|---|---|
| Application Notes | Recommended dilutions: FACS: 1/100. ICC: Use at an assay dependent dilution. IHC: Use at a concentration of 2 μg/ml. IP: Use at a concentration of 12.5 μg/ml. WB: Use at a concentration of 0.5 μg/ml. Predicted molecular weight: 48 kDa. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None Constituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2 |
