Application
Western blot analysis of calsequestrin in canine skeletal muscle extract.Application
Immunofluorescent analysis of Calsequestrin in HeLa Cells. Calsequestrin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Calsequestrin antibody [VIIID12] at a dilution of 1:100 over night at 4?C, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.Application
Immunohistochemistry was performed on normal deparaffinized human heart tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with or without Calsequestrin antibody [VIIID12] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Product Name | Calsequestrin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [VIIID12] to Calsequestrin |
| Immunogen | Purified rabbit skeletal muscle sarcoplasmic reticulum. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | VIIID12 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PIPWB |
| ICC/IF: 1:50 - 1:500: |
|
| Species Reactivity | HumanMouseChickenDogPigRatRabbit |
| Concentration | 1mg/ml |
| Gene Synonyms | CASQ1 calsequestrin 1 (fast-twitch skeletal muscle) |
|---|---|
| Target | Calsequestrin |
| Tissue Specificity | This antibody recognizes calsequestrin in both type I (slow) and type II (fast) skeletal muscle tissues. |
Application
Western blot analysis of calsequestrin in canine skeletal muscle extract.
Application
Immunofluorescent analysis of Calsequestrin in HeLa Cells. Calsequestrin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Calsequestrin antibody [VIIID12] at a dilution of 1:100 over night at 4?C, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.
Application
Immunohistochemistry was performed on normal deparaffinized human heart tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with or without Calsequestrin antibody [VIIID12] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Application Notes | ICC/IF: 1:50 - 1:500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | Ascites containing 0.05 % sodium azide |
