Application
Western blot of rat cortex lysate showing phospho-specific immunolabeling of the ~50k alpha-subunit and ~60k beta-subunit of the CaM Kinase II protein phosphorylated at Thr 286 using CaMKII (phospho Thr286) antibody .| Product Name | CaMKII alpha (phospho Thr286) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CaMKII alpha (phospho Thr286)CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 conjugated to KLH |
| Modification | p-Thr286 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | phosphoCaMKII (pT286) phosphoCaMKII (pThr286) phospho CaMKII (pThr286) CAMK2A/B EC2.7.11.17 KIAA0968 CAMKA CAMK2A phospho-CaMKII (pT286) phospho CaMKII (pT286) phospho-CaMKII (pThr286) EC 2.7.11.17 CaMKII (phospho T286) CaMKII (phospho Thr286) |
|---|---|
| Target | CaMKII (phospho Thr286) |
| Molecular Weight(MW) | 50/60 (note) |
| Cellular Localization | Postsynaptic lipid rafts. |
Application
Western blot of rat cortex lysate showing phospho-specific immunolabeling of the ~50k alpha-subunit and ~60k beta-subunit of the CaM Kinase II protein phosphorylated at Thr 286 using CaMKII (phospho Thr286) antibody .| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol. |
