Application
Rat brain lysates were either left untreated (1-4) or treated with lambda (l) phosphatase (5). Lysates were then incubated with CaM Kinase II Alpha [pT286] Polyclonal Antibody, Rabbit, following prior incubation.| Product Name | CaMKII alpha (phospho Thr286) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CaMKII alpha ( phospho T286 )Calcium/calmodulin-dependent protein kinase II alpha (CaM Kinase II alpha) is a 50 kDa member of CaM Kinase II family of serine-threonine kinases that transduce Ca2+ signals to several target proteins, including ion channels and transcription activators. CaM Kinase II is predominantly expressed in two isoforms in the brain: alpha and beta. CaM Kinase II plays an important role in neuronal plasticity and memory formation, and exerts both calcium-calmodulin-dependent and -independent activities. Autophosphorylation of CaM Kinase II alpha on threonine 286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state, and is required for various cellular functions including hippocampal long-term potentiation (LTP), special learning, and hippocampus-dependent memory. |
| Immunogen | Synthetic phosphopeptide derived from a region of human CaM Kinase II alpha that contains threonine 286. |
| Modification | p-Thr286 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. Detects a band of approximately 50 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CAMK2A Q9UQM7 CaMKII alpha 114078 KIAA0968 CAMKA 815 CaMKII alpha (phospho T286) CaMKII alpha (phospho Thr286) CaMKII alpha (pT286) CaMKII alpha (pThr286) CaMKII alpha phospho T286 CaMKII alpha phospho Thr286 Phospho CaMKII alpha (T286) Phos |
|---|---|
| Target | CaMKII alpha |
Application
Rat brain lysates were either left untreated (1-4) or treated with lambda (l) phosphatase (5). Lysates were then incubated with CaM Kinase II Alpha [pT286] Polyclonal Antibody, Rabbit, following prior incubation.| Positive Control | Rat brain lysate |
|---|---|
| Application Notes | WB: Use at a dilution of 1/1000. Detects a band of approximately 50 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
