Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPZA1 monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPZA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPZA1.| Product Name | CAPZA1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [7C8] to CAPZA1F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. |
| Immunogen | Full length human recombinant protein of human CAPZA1 (NP_006126) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7C8 |
| Host Species | Mouse |
| Tested Applications | WB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CAPPA1 CAPZA1 CAPZ CAZ1 |
|---|---|
| Target | CAPZA1 |
| Tissue Specificity | is specific for human CAPZA1 (NP_006126). |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPZA1 monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPZA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPZA1.| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
