WB
Western blot analysis of CAPZA1 on different lysates with Mouse anti-CAPZA1 antibody at 1/5,000 dilution. Lane 1: K-562 cell lysate, Lane 2: 293T cell lysate, Lane 3: Jurkat cell lysate, Lane 4: HeLa cell lysate, Lane 5: HepG2 cell lysate, Lane 6: A549 cell lysate, Lane 7: NIH/3T3 cell lysate, Lane 8: RAW264.7 cell lysate, Lane 9: C2C12 cell lysate, Lane 10: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-CAPZA1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling CAPZA1 with Mouse anti-CAPZA1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CAPZA1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (594) were used as the secondary antibody at 1/1,000 dilution.| Product Name | CAPZA1 Recombinant Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human CAPZA1 aa 60-248 / 286. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000 IHC:1:1000 ICC/IF:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CAPZA1 |
|---|---|
| Gene Synonyms | CAPZ CAZ1 CAPPA1 |
| Gene Full Name | capping actin protein of muscle Z-line subunit alpha 1 |
| Gene Summary | CAPZA1 is a member of the F-actin capping protein alpha subunit family. This gene encodes the alpha subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments. [provided by RefSeq, Jul 2008] |
| Alternative Names | Cap Z antibody Cappa 1 antibody Cappa1 antibody Capping protein (actin filament) muscle Z line alpha 1 antibody Capping protein alpha 1 antibody Capping protein muscle Z line alpha 1 antibody CapZ alpha 1 antibody CapZ alpha-1 antibody CAPZ antibody CAPZA 1 antibody Cap Z antibody Cappa 1 antibody Cappa1 antibody Capping protein (actin filament) muscle Z line alpha 1 antibody Capping protein alpha 1 antibody Capping protein muscle Z line alpha 1 antibody CapZ alpha 1 antibody CapZ alpha-1 antibody CAPZ antibody CAPZA 1 antibody Capza1 antibody CAZ 1 antibody CAZ1 antibody CAZA1_HUMAN antibody F actin capping protein alpha 1 subunit antibody F-actin-capping protein subunit alpha-1 antibody |
| Molecular Weight(MW) | 33 kDa |
| Cellular Localization | Cytoskeleton. |
WB
Western blot analysis of CAPZA1 on different lysates with Mouse anti-CAPZA1 antibody at 1/5,000 dilution. Lane 1: K-562 cell lysate, Lane 2: 293T cell lysate, Lane 3: Jurkat cell lysate, Lane 4: HeLa cell lysate, Lane 5: HepG2 cell lysate, Lane 6: A549 cell lysate, Lane 7: NIH/3T3 cell lysate, Lane 8: RAW264.7 cell lysate, Lane 9: C2C12 cell lysate, Lane 10: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-CAPZA1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling CAPZA1 with Mouse anti-CAPZA1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CAPZA1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (594) were used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:5000 IHC:1:1000 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
