Application
Detection ofcarboxypeptidease B2 (TAFI)with [13H4] in western blotting (non reducing conditions).Application
Titration curves of carboxypeptidase B2 in plasma using antibody pair; capture 200ng/welland conjugated to HRP. Incubated at room temperature.| Product Name | Carboxypeptidase B2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [13H4] to Carboxypeptidase B2This protein belongs to a family of Zn-containing metallocarboxypeptidases specific to C-terminal lysine and arginine residues. It circulates in plasma as a zymogen with molecular weigh of 55 kDa (401 amino acid residues; pI 5.0, Fig. 32). Being activated by thrombin-thrombomodulin complex during blood coagulation, it exerts carboxypeptidase activity. Activated it removes C-terminal lysine residues from fibrin, which are necessary for plasminogen binding to fibrin. This prevents plasminogen from activation into plasmin and retards the lysis of a fibrin clot. The concentration in plasma of healthy people is 5 10 μg/ml. High plasma levels were found in patients with stable angina pectoris and angiographically verified coronary artery disease. Elevated concentration in blood is considered as a risk factor for venous thrombosis. A deficiency might contribute to the severity of bleeding disorders in hemophilias A and B, and decreased levels are found in chronic liver disease. |
| Immunogen | Full length native protein (purified) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 13H4 |
| Host Species | Mouse |
| Tested Applications | EIAELISAWB |
| ELISA: Use at an assay dependent dilution. EIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 50.8 kDa. Can be paired for ELISA with this antibody as detection and GTX14756 Mouse monoclonal [13D5] to Carboxypeptidase B2 or GTX14763 Mouse monoclonal [15A10] to Carboxypeptidase B2 as capture. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | CPB2 Carboxypeptidase B2 603101 CPU 1361 PCPB Q96IY4 TAFI |
|---|---|
| Target | Carboxypeptidase B2 |
Application
Detection ofcarboxypeptidease B2 (TAFI)with [13H4] in western blotting (non reducing conditions).
Application
Titration curves of carboxypeptidase B2 in plasma using antibody pair; capture 200ng/welland conjugated to HRP. Incubated at room temperature.| Application Notes | ELISA: Use at an assay dependent dilution. EIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 50.8 kDa. Can be paired for ELISA with this antibody as detection and GTX14756 Mouse monoclonal [13D5] to Carboxypeptidase B2 or GTX14763 Mouse monoclonal [15A10] to Carboxypeptidase B2 as capture. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.4 |
