Application
Both the antiserum and IgG fractions of anti-Carboxypeptidase Y (Baker's Yeast) are shown to detect under reducing conditions of SDS-PAGE the 61,000 dalton enzyme in cellular extracts. Approximately 10 µg of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-anti-Rabbit IgG (H&L) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.| Product Name | Carboxypeptidase Y antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Carboxypeptidase YInvolved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate. |
| Immunogen | Carboxypeptidase Y [Baker's Yeast] |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | S.cerevisiae |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LBC1 PRC1 |
|---|---|
| Target | Carboxypeptidase Y |
| Cellular Localization | Lysosome-like vacuoles. |
Application
Both the antiserum and IgG fractions of anti-Carboxypeptidase Y (Baker's Yeast) are shown to detect under reducing conditions of SDS-PAGE the 61,000 dalton enzyme in cellular extracts. Approximately 10 µg of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-anti-Rabbit IgG (H&L) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
