Application
Troponin I (cardiac) antibody [N1C3] detects Troponin I (cardiac) protein at cytosol on rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. Troponin I (cardiac) antibody [N1C3] dilution: 1:500.Application
Cardiac Troponin I antibody [N1C3] detects Cardiac Troponin I protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Cardiac Troponin I antibody [N1C3] dilution: 1:500. Scale bar = M μm.Application
Sample (30 µg of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HepG2, using Cardiac Troponin I antibody at 1:200 dilution.Application
Sample (50 µg of whole cell lysate) A: mouse heart 12% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Cardiac Troponin I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cardiac Troponin I (troponin I type 3 (cardiac))Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq] |
| Immunogen | Full length human Cardiac Troponin I Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Cat, Pig, BovineDog (95%), Cat (94%), Pig (93%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CMD1FF antibody CMD2A antibody CMH7 antibody MGC116817 antibody RCM1 antibody TNNC1 antibody cTnI antibody TNNI3 antibody troponin I cardiac muscle antibody troponin I type 3 (cardiac) antibody Troponin I (cardiac) antibody |
|---|---|
| Molecular Weight(MW) | 24 kDa (note) |
Application
Troponin I (cardiac) antibody [N1C3] detects Troponin I (cardiac) protein at cytosol on rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. Troponin I (cardiac) antibody [N1C3] dilution: 1:500.
Application
Cardiac Troponin I antibody [N1C3] detects Cardiac Troponin I protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Cardiac Troponin I antibody [N1C3] dilution: 1:500. Scale bar = M μm.
Application
Sample (30 µg of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HepG2, using Cardiac Troponin I antibody at 1:200 dilution.
Application
Sample (50 µg of whole cell lysate) A: mouse heart 12% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HepG2 , mouse heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
