Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: mouse heart 12% SDS PAGE diluted at 1:5000Application
Cardiac Troponin I antibody [N1C3-3] detects Cardiac Troponin I protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cardiac Troponin I protein stained by Cardiac Troponin I antibody [N1C3-3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Cardiac Troponin I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cardiac Troponin I (troponin I type 3 (cardiac))Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cardiac Troponin I. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMousePig |
| Predicted Reactivity | Rat, Dog, Cat, BovineRat (93%), Dog (97%), Cat (97%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CMD1FF antibody CMD2A antibody CMH7 antibody MGC116817 antibody RCM1 antibody TNNC1 antibody cTnI antibody TNNI3 antibody troponin I cardiac muscle antibody troponin I type 3 (cardiac) antibody Troponin I (cardiac) antibody |
|---|---|
| Molecular Weight(MW) | 24 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: mouse heart 12% SDS PAGE diluted at 1:5000
Application
Cardiac Troponin I antibody [N1C3-3] detects Cardiac Troponin I protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cardiac Troponin I protein stained by Cardiac Troponin I antibody [N1C3-3] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , Raji , mouse heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
