Application
ICC/IF analysis of HeLa cells transiently transfected with flag-tagged Cas9 using Cas9 antibody [7A9] at a dilution of 2 μg/ml (green) and DAPI (blue).Application
IP analysis was performed with lysate from HEK293T cells expressing Flag-tagged S. pyogenes Cas9 and either Cas9 antibody [7A9] followed by incubation with a 1:1 mixture of protein A/G sepharose beads (Cas9 Sup) or Cas9 antibody [7A9] crosslinked with a 1:1 mixture of protein A/G sepharose beads (Cas9 X-link). The precipitates were detected by the same antibody. Asterisk indicates IgG heavy chain.| Product Name | Cas9 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [7A9] to Cas9 |
| Immunogen | N-Terminal domain of Cas9. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7A9 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| WB:1:500~1:1000 IP:1:100~1:500 ICC/IF:1:100~1:500 |
|
| Concentration | 1mg/ml |
| Purification | Protein G |
| Target | Cas9 |
|---|---|
| Molecular Weight(MW) | 124 kDa |
| Tissue Specificity | This antibody recognises both Cas9 and dCas9 (nuclease deficient Cas9) |
| Light Chain Type | kappa |
| SwissProt ID | J7RUA5 |
|---|
Application
ICC/IF analysis of HeLa cells transiently transfected with flag-tagged Cas9 using Cas9 antibody [7A9] at a dilution of 2 μg/ml (green) and DAPI (blue).
Application
IP analysis was performed with lysate from HEK293T cells expressing Flag-tagged S. pyogenes Cas9 and either Cas9 antibody [7A9] followed by incubation with a 1:1 mixture of protein A/G sepharose beads (Cas9 Sup) or Cas9 antibody [7A9] crosslinked with a 1:1 mixture of protein A/G sepharose beads (Cas9 X-link). The precipitates were detected by the same antibody. Asterisk indicates IgG heavy chain.| Application Notes | WB:1:500~1:1000 IP:1:100~1:500 ICC/IF:1:100~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for 1 year at 2-8°C from date of receipt. |
| Storage Buffer | Buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
