Application
Western blot analysis of caspase-2 in untreated (Lane 1,2) and treated (Lane 3,4) cell lysate.| Product Name | Caspase 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase 2Caspases play a key role in apoptosis. Similar as other caspases, caspase-2 is also synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis. Caspase-2 mRNA is alternatively spliced. The larger mRNA species encoding a product of 435 amino acids is known as ICH-1L. The smaller mRNA species encoding a protein of 312 amino acids is named ICH-1S. Over expression of ICH-1L induces apoptosis, while over expression of ICH-1S suppresses Rat-1 cell death induced by serum deprivation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (1-2 µg/ml). However, the optimal conditions should be determined individually. Detects the full length and small subunit of caspase-2. In human samples, it also detects a 30 kDa intermeadiate fragment: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NEDD2 ICH1L/1S ICH1L ICH1 EC 3.4.22.55 EC3.4.22.55 NEDD-2 ICH-1L/1S CASP2 CASP-2 ICH-1L Caspase2 NEDD 2 CASP 2 ICH 1L ICH 1L/1S |
|---|---|
| Target | Caspase 2 |
| Tissue Specificity | This antibody is specific for Caspase-2. |
Application
Western blot analysis of caspase-2 in untreated (Lane 1,2) and treated (Lane 3,4) cell lysate.| Application Notes | Western blotting (1-2 µg/ml). However, the optimal conditions should be determined individually. Detects the full length and small subunit of caspase-2. In human samples, it also detects a 30 kDa intermeadiate fragment: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
