Application
Western blot analysis of Caspase-5 in rat kidney tissue lysate. Caspase-5 intermediate (30 kDa) and full cleaved (20 kDa) fragments were detected.| Product Name | Caspase 5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase 5Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-5 is also known as Ich-3 and ICErel-III and can proteolytically processed by granzyme B. Over expression of caspase-5 induces apoptosis in Rat-1 and HeLa cells, which can be inhibited by CrmA and Bcl-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4ag/ml). However, the optimal conditions should be determined individually. The affinity purified antibody preferentially detects the intermediate (32 kDa) and the fully (20 kDa) cleaved caspase-5.: |
|
| Species Reactivity | HumanMouseMonkeyRatHamsterBovine |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ICH3 ICH3 protease CASP-5 TY protease ICH-3 protease CASP5 Caspase5 CASP 5 ICH 3 protease |
|---|---|
| Target | Caspase 5 |
| Tissue Specificity | This antibody is specific for Caspase-5. |
Application
Western blot analysis of Caspase-5 in rat kidney tissue lysate. Caspase-5 intermediate (30 kDa) and full cleaved (20 kDa) fragments were detected.| Application Notes | Western blotting (0.5-4ag/ml). However, the optimal conditions should be determined individually. The affinity purified antibody preferentially detects the intermediate (32 kDa) and the fully (20 kDa) cleaved caspase-5.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal |
