Application
Western blot analysis of Caspase-5 in (A) human spleen tissue and (B) human thymus lysate with Caspase-5 antibody at 1 μg/mL.Application
Western blot analysis of Caspase-5 in Ramos cells with Caspase-5 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Product Name | Caspase-5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Caspase-5 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-5 can interact with caspase-1; both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1β. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma.1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74. |
| Immunogen | Caspase-5 antibody was raised against a 16 amino acid synthetic peptide from the amino-terminus of human Caspase-5. The immunogen is located within amino acids 40 - 90 of Caspase-5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Casp-5 antibody can be used for the detection of Caspase-5 by Western blot at 0.5 to 2 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP5 |
|---|---|
| Alternative Names | Caspase-5 Antibody: ICH-3 ICEREL-III ICE(rel)III ICH3 Caspase-5 Protease ICH-3 CASP-5 |
| Molecular Weight(MW) | Predicted: 48 kDa Observed: 48 kDa |
Application
Western blot analysis of Caspase-5 in (A) human spleen tissue and (B) human thymus lysate with Caspase-5 antibody at 1 μg/mL.
Application
Western blot analysis of Caspase-5 in Ramos cells with Caspase-5 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Application Notes | Casp-5 antibody can be used for the detection of Caspase-5 by Western blot at 0.5 to 2 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Caspase-5 Antibody is supplied in PBS containing 0.02% sodium azide. |
