| Product Name | Caspase 6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3F52] to Caspase 6Caspases play a major role in the transduction of the apoptotic signal and execution of apoptosis in mammalian cells. Caspase 3 (CPP32) and Caspase 6 (Mch2) are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis inducing stimuli and in all cell lines analyzed. Both CPP32 and Mch2 are present in apoptotic cells as multiple active species. Caspase 6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei. |
| Immunogen | Recombinant caspase 6 prodomain (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3F52 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| IHC-P: Use at a concentration of 1 - 2 μg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temp for 20 minIP: Use at a concentration of 2 μg/ml. Denatured verified. Use Protein G. WB: Use at a concentration of 1 μg/ml for 2 hours at room temp. Detects a band of approximately 34 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | Caspase 6 P55212 CASP6 MCH2 839 Caspase6 601532 |
|---|---|
| Target | Caspase 6 |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
| Positive Control | Jurkat cells Tonsil |
|---|---|
| Application Notes | IHC-P: Use at a concentration of 1 - 2 μg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temp for 20 minIP: Use at a concentration of 2 μg/ml. Denatured verified. Use Protein G. WB: Use at a concentration of 1 μg/ml for 2 hours at room temp. Detects a band of approximately 34 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide. Constituents: 0.2% BSA, 10mM PBS. pH 7.4 |
