| Product Name | Caspase 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal to Caspase 8 ( FLICE )Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the proform of the Caspase 8 protein. Recruitment of Caspase 8 to the Fas receptor results in oligomerization of the Caspase 8 protein, which in turn drives its autoactivation through ""self-cleavage"". Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby commiting the cell to undergo apoptosis. Reacts with Jurkat cells and Tonsil. |
| Immunogen | The epitope corresponding to amino acids 217-350 mapping within the caspase-8 p18 subunit of human origin. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| For IHC-P: Use at a dilution of 1:100. For IP: Use at a dilution of 1:200. For WB: Use at a diltuion of 1:1000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Q14790 MGC78473 MACH Caspase8 114550 CASP8 MCH5 Caspase 8 841 FLICE |
|---|---|
| Target | Caspase 8 |
| Tissue Specificity | It is recommended for detection of the p18 subunit and precursor of caspase-8 of mouse, rat and human origin. |
| Cellular Localization | Cytoplasmic |
| Positive Control | Jurkat Cells, Stomach |
|---|---|
| Application Notes | For IHC-P: Use at a dilution of 1:100. For IP: Use at a dilution of 1:200. For WB: Use at a diltuion of 1:1000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM Phosphate-buffered saline containing 0.2% BSA and 0.1% sodium azide |
