| Product Name | Caspase 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Caspase 8Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse. |
| Immunogen | Synthetic peptide: SDSPREQDSESQTLDK conjugated to KLH, corresponding to N terminal amino acids 209-224 of Human Caspase 8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000 (determined by using a whole cell extract of the human Burkitt lymphoma Raji cell line). Expected bands at: 55 kDa (human caspase 8a) and 54 kDa (caspase 8b). Possible bands at 30-32 kDa (caspase 8 MACH beta 3/beta 4 isoforms). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Q14790 MGC78473 MACH Caspase8 114550 CASP8 MCH5 Caspase 8 841 FLICE |
|---|---|
| Target | Caspase 8 |
| Tissue Specificity | Anti-Caspase 8 (SK-16) recognizes human caspase 8a and caspase 8b by immunoblotting. The product may also detect the caspase 8 MACH beta 3/beta 4 isoforms. |
| Cellular Localization | Cytoplasmic |
| Positive Control | Whole cell extract of the human Burkitt lymphoma Raji cell line |
|---|---|
| Application Notes | WB: Use at a dilution of 1/1000 (determined by using a whole cell extract of the human Burkitt lymphoma Raji cell line). Expected bands at: 55 kDa (human caspase 8a) and 54 kDa (caspase 8b). Possible bands at 30-32 kDa (caspase 8 MACH beta 3/beta 4 isoforms). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
