| Product Name | Caspase 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Caspase 8Caspase 8 has an N-terminal domain with sequence homology to the death effector domain (DED) of FADD. Caspase 8 binds to DED of FADD. Caspase 8 is activated by association with Fas / FADD death inducing signaling complex to release two active subunits, p18 and p10 into the cytosol, where they activate other Caspases amplifying the apoptotic signal. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| Immunohistochemistry: use at a dilution of 1/50 - 1/100 for 30mins at RT (ABC method). Optimal dilutions / concentrations should be determined by the end user. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with the IHC staining protocol.: |
|
| Species Reactivity | HumanMouseMonkeyRatBovine |
| Concentration | 1mg/ml |
| Gene Synonyms | Q14790 MGC78473 MACH Caspase8 114550 CASP8 MCH5 Caspase 8 841 FLICE |
|---|---|
| Target | Caspase 8 |
| Tissue Specificity | This antibody reacts with a 55 kDa protein. |
| Cellular Localization | Cytoplasmic |
| Positive Control | Stomach |
|---|---|
| Application Notes | Immunohistochemistry: use at a dilution of 1/50 - 1/100 for 30mins at RT (ABC method). Optimal dilutions / concentrations should be determined by the end user. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with the IHC staining protocol.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS with sodium azide |
